COVID-19 Vaccine Candidate Shows Promise

PITTSBURGH  –  University of Pittsburgh School of Medicine  scientists today announced a potential vaccine against SARS-CoV-2, the new co...


PITTSBURGH – University of Pittsburgh School of Medicine scientists today announced a potential vaccine against SARS-CoV-2, the new coronavirus causing the COVID-19 pandemic. When tested in mice, the vaccine, delivered through a fingertip-sized patch, produces antibodies specific to SARS-CoV-2 at quantities thought to be sufficient for neutralizing the virus. 

The paper appeared today in EBioMedicine, which is published by The Lancet, and is the first study to be published after critique from fellow scientists at outside institutions that describes a candidate vaccine for COVID-19. The researchers were able to act quickly because they had already laid the groundwork during earlier coronavirus epidemics.   

“We had previous experience on SARS-CoV in 2003 and MERS-CoV in 2014. These two viruses, which are closely related to SARS-CoV-2, teach us that a particular protein, called a spike protein, is important for inducing immunity against the virus. We knew exactly where to fight this new virus,” said co-senior author Andrea Gambotto, M.D., associate professor of surgery at the Pitt School of Medicine. “That’s why it’s important to fund vaccine research. You never know where the next pandemic will come from.”

“Our ability to rapidly develop this vaccine was a result of scientists with expertise in diverse areas of research working together with a common goal,” said co-senior author Louis Falo, M.D., Ph.D., professor and chair of dermatology at Pitt’s School of Medicine and UPMC.

Compared to the experimental mRNA vaccine candidate that just entered clinical trials, the vaccine described in this paper — which the authors are calling PittCoVacc, short for Pittsburgh Coronavirus Vaccine — follows a more established approach, using lab-made pieces of viral protein to build immunity. It’s the same way the current flu shots work.  

Falo Gambotto SARS 17 releaseThe researchers also used a novel approach to deliver the drug, called a microneedle array, to increase potency. This array is a fingertip-sized patch of 400 tiny needles that delivers the spike protein pieces into the skin, where the immune reaction is strongest. The patch goes on like a Band-Aid and then the needles — which are made entirely of sugar and the protein pieces — simply dissolve into the skin. 

“We developed this to build on the original scratch method used to deliver the smallpox vaccine to the skin, but as a high-tech version that is more efficient and reproducible patient to patient,” Falo said. “And it’s actually pretty painless — it feels kind of like Velcro.”

The system also is highly scalable. The protein pieces are manufactured by a “cell factory” — layers upon layers of cultured cells engineered to express the SARS-CoV-2 spike protein — that can be stacked further to multiply yield. Purifying the protein also can be done at industrial scale. Mass-producing the microneedle array involves spinning down the protein-sugar mixture into a mold using a centrifuge. Once manufactured, the vaccine can sit at room temperature until it’s needed, eliminating the need for refrigeration during transport or storage.

Falo Gambatto SARS play“For most vaccines, you don’t need to address scalability to begin with,” Gambotto said. “But when you try to develop a vaccine quickly against a pandemic that’s the first requirement.”

When tested in mice, PittCoVacc generated a surge of antibodies against SARS-CoV-2 within two weeks of the microneedle prick. 

Those animals haven’t been tracked long term yet, but the researchers point out that mice who got their MERS-CoV vaccine produced a sufficient level of antibodies to neutralize the virus for at least a year, and so far the antibody levels of the SARS-CoV-2 vaccinated animals seem to be following the same trend. 

Importantly, the SARS-CoV-2 microneedle vaccine maintains its potency even after being thoroughly sterilized with gamma radiation — a key step toward making a product that’s suitable for use in humans.  

The authors are now in the process of applying for an investigational new drug approval from the U.S. Food and Drug Administration in anticipation of starting a phase I human clinical trial in the next few months. 

“Testing in patients would typically require at least a year and probably longer,” Falo said. “This particular situation is different from anything we’ve ever seen, so we don’t know how long the clinical development process will take. Recently announced revisions to the normal processes suggest we may be able to advance this faster.”

Additional authors on the study are Eun Kim, Geza Erdos, Ph.D., Shaohua Huang, Thomas Kenniston, Stephen Balmert, Ph.D., Cara Donahue Carey, Michael Epperly, Ph.D., William Klimstra, Ph.D., and Emrullah Korkmaz, Ph.D., all of Pitt; and Bart Haagmans, of Erasmus Medical Center.

Funding for this study was provided by National Institute of Allergy and Infectious Diseases grant R21-AI114264, National Institute of Arthritis and Musculoskeletal and Skin Diseases grants R01-AR074285, R01-AR071277 and R01-AR068249, and National Cancer Institute grant T32-CA175294.

When the embargo lifts, UPMC and Pitt will conduct a virtual press conference where the authors will answer questions from reporters. There is limited availability for reporter participation. Please contact Erin Hare (HareE@upmc.edu) by 5pm on Wednesday, April 1, if you’d like to participate.

https://www.upmc.com/media/news/040220-falo-gambotto-sars-cov2-vaccine

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आडूबान,1,जानवरों की अभिव्यक्ति,1,जीवन और जंग,1,जीवन की उत्‍पत्ति,1,जैव विविधता वर्ष,1,जैव शवदाह,1,ज्योतिष,1,ज्योतिष और अंधविश्वास,2,झारखण्‍ड,1,टिंडल प्रभाव,1,टीलोमियर,1,टीवी और स्‍वास्‍थ्‍य,1,टीवी के दुष्‍प्रभाव,1,टेक्‍नालॉजी,1,टॉप 10 ब्लॉगर,1,डा0 अब्राहम टी कोवूर,1,डा0 ए0 पी0 जे0 अब्दुल कलाम,1,डाइनामाइट,1,डाटा सेंटर,1,डिस्कस,1,डी•एन•ए• की खोज,3,डीप किसिंग,1,डॉ मनोज पटैरिया,1,डॉ. के.एन. पांडेय,1,डॉ० मिश्र,1,ड्रग एडिक्‍ट,1,ड्रग्स,1,ड्रग्‍स की लत,1,तम्बाकू,1,तम्‍बाकू के दुष्‍प्रभाव,1,तम्बाकू निषेध,1,तर्कशास्त्र,1,ताँत्रिक क्रियाएँ,1,थर्मोइलेक्ट्रिक जेनरेटर,1,थ्री ईडियट्स के फार्मूले,1,दर्दनाशी,1,दर्शन,1,दर्शन लाल बवेजा,3,दिल की बीमारी,1,दिव्‍य शक्ति,1,दुरबीन,1,दूरानुभूति,1,दोहरे मानदण्ड,1,धरोहर,1,धर्म,2,धातु विज्ञान,1,धार्मिक पाखण्ड,1,धुम्रपान और याद्दाश्‍त,1,धुम्रपान के दुष्‍प्रभाव,1,धूल-मिट्टी,1,नई खोजें,1,नन्हे आविष्कार,1,नमक,1,नवाचारी भौतिकी,1,नशीली दवाएं,1,नाइट शिफ्ट के दुष्‍प्रभाव,1,नारायणमूर्ति,1,नारी-मुक्ति,1,नींद और बीमारियां,1,नींद न आने के कारण,1,नेत्रदान और 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पम्प,1,ब्रह्मण्‍ड,1,ब्रह्मा,1,ब्रह्माण्‍ड,1,ब्रह्माण्‍ड के रहस्‍य,1,ब्रह्मान्ड,1,ब्रेन म्‍यूजिक,1,ब्लॉग लेखन,1,ब्लॉग लेखन के द्वारा विज्ञान संचार,2,ब्लॉगिंग का महत्व,1,भारतीय आयुर्विज्ञान अनुसंधान परिषद,1,भारतीय विज्ञान कथा लेखक समिति,1,भारतीय वैज्ञानिक,2,भारतीय शोध,1,भूकंप के झटके,1,भूकम्‍प,1,भूगर्भिक हलचलें,1,मंगल,1,मधुमेह और खानपान,1,मनजीत सिंह बोपाराय,1,मनीष मोहन गोरे,1,मनीष वैद्य,1,मनु स्मृति,1,मनोरंजक गणित,1,महिला दिवस,1,माचू-पिचू,1,मानव शरीर,1,माया,1,मारिजुआना,1,मासिक धर्म,1,मिल्‍की वे,1,मिशन स्टारडस्ट-नेक्स,1,मीठी गपशप,1,मीमांसा,1,मुख कैंसर,1,मृत सागर,1,मेघ राज मित्र,1,मेडिकल रिसर्च,1,मेरी शैली,1,मैथेमेटिक्स ओलम्पियाड,1,मैरी क्‍यूरी,2,मैरीन इनोवेटिव टेक्नोलॉजीज लि,1,मोटापा,1,मोबाईल के नुकसान,1,मौसम,1,यजुर्वेद,1,युवा अनुसंधानकर्ता पुरष्कार,1,यूरी गागरिन,1,योगेन्द्र पाल,1,योगेश,1,योगेश रे,1,रक्षा उपकरण,1,राईबोसोम,1,रूप गठन,1,रेडियो टोमोग्राफिक इमेजिंग,1,रैबीज,1,रोचक रोमांचक अंटार्कटिका,4,रोबोटिक्स,1,लखनऊ,1,लादेन,1,लालन-पालन,1,लिनक्स,1,लिपरेशी,1,लीप इयर,1,लेड,1,लॉ ऑफ ग्रेविटी,1,लोक विज्ञान,1,लौह स्तम्भ,1,वजन घटाने का आसान तरीका,1,वाई गुणसूत्र,1,वायु प्रदुषण,1,वाशो,1,विज्ञान,1,विज्ञान कथा,3,विज्ञान कथा सम्मेलन,1,विज्ञान के खेल,2,विज्ञान चुटकले,1,विज्ञान तथा प्रौद्यौगिकी,1,विज्ञान प्रगति,1,विज्ञान ब्लॉग,1,विज्ञापन,1,विटामिनों के वहम,1,विद्युत,1,विवेकानंद,1,विवेचना-व्याख्या,1,विश्व नि-तम्बाकू दिवस,1,विश्व पर्यावरण दिवस,1,विश्व भूगर्भ जल दिवस,1,विष्णु,1,वीडियो,1,वीडियो बुक,1,वैज्ञानिक दृष्टिकोण,1,वैद्य अश्विनी कुमार,1,वोस्तोक,1,व्‍यायाम के लाभ,1,व्हेलॉसिटी,1,शिव,1,शुक्र पारगमन,1,शुगर के दुष्‍प्रभाव,1,शून्य,1,शोध परिणाम,1,शोधन,1,श्रृष्टि का अंत,1,सं. राष्ट्रसंघ,1,सकारात्‍मक सोच का जादू,1,संक्रमण,1,संख्या,1,संजय ग्रोवर,1,संज्ञात्मक पक्षी विज्ञान,1,सटीक व्‍यायाम,1,संत बलबीर सिंह सीचेवाल,1,सत्यजित रे,1,समय की बरबादी को रोचकने के उपाय,1,समाज और हम,1,समुद्र,1,संयोग,1,सर चन्द्रशेखर वेंकट रमन राष्ट्रीय विज्ञान दिवस,1,सर्प संसार,1,साइकोलोजिस्ट,1,साइनस उपचार,1,साइंस ब्लागर्स मीट,1,साइंस ब्लागर्स मीट.,2,साइंस ब्लॉग कार्यशाला,2,साइंस ब्लॉगर्स असोसिएशन अवार्ड,1,साइंस ब्लॉगर्स असोसिएशन रजिस्ट्रेशन,1,साइंस ब्लॉगर्स ऑफ दि ईयर अवार्ड,1,साइंस ब्लोगिंग,1,साईकिल,1,सामवेद,1,सामाजिक अभिशाप,1,सामाजिक चेतना,1,साहित्यिक चोरी,2,सिगरेट छोड़ें,1,सी. एस. आई. आर.,1,सी.वी.रमण विज्ञान क्लब,1,सीजेरियन ऑपरेशन,1,सुपर अर्थ,1,सुपर कम्प्यूटर,1,सुरक्षित ब्लॉगिंग,1,सूर्यग्रहण,2,सृष्टि व जीवन,3,सेक्स रेशियो,1,सेहत की देखभाल,1,सोशल नेटवर्किंग,1,स्टीफेन हाकिंग,1,स्पेन,1,स्मृति,1,स्वर्ण अनुपात,1,स्वाईन-फ्लू,1,स्वास्थ्य,2,स्‍वास्‍थ्‍य और खानपान,1,स्वास्थ्य चेतना,3,हमारे वैज्ञानिक,4,हरित क्रांति,1,हंसी के फायदे,1,हाथरस कार्यशाला,1,हिंद महासागर,1,हृदय रोग,1,होलिका दहन,1,ह्यूमन रोबोट,1,
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Science Bloggers' Association: COVID-19 Vaccine Candidate Shows Promise
COVID-19 Vaccine Candidate Shows Promise
https://www.upmc.com/-/media/upmc/media/publishingimages/2020/march/falo-gambotto-sars-17-release.jpg?h=200&w=350&la=en&hash=AE5455E340401BB696F5CE8865A1EC76
Science Bloggers' Association
https://blog.scientificworld.in/2020/04/covid-19-vaccine-candidate-shows-promise.html
https://blog.scientificworld.in/
https://blog.scientificworld.in/
https://blog.scientificworld.in/2020/04/covid-19-vaccine-candidate-shows-promise.html
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